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Aberration-corrected and label-free scanning endomicroscopy using GRIN lens
2019 IBS AOI Conference GRIN lenses are essential probe for miniature endoscope and deep tissue imaging. It can be made very thin and their length can be easily adjusted by combination of relay lenses, it is possible to perform minimally invasive imaging that minimized the damage to tissue during imaging. However, GRIN lenses have inherent aberratio...
RegDate
2020-06-29
Hit
2408
Mid-IR Photothermal Microscopy
2019 IBS AOI Conference There have been various imaging techniques applying infrared (IR) source for its advantages such as chemical selectivity, non-destructiveness, and label-free character. Recently, one of promising imaging techniques utilizing IR source is an infrared photothermal microscopy. 1,2 In this IR technique, a mid-IR excitation of spec...
RegDate
2020-06-26
Hit
2396
Real-time observation of the individual focal adhesion dynamics and cytoskeletons via iSCAT microscopy
2019 IBS AOI Conference Focal adhesion complexes (FAs) are large, dynamic protein systems that form mechanical links between cytoskeletal actin fibers and extracellular matrix. To date, our current understanding in their structures and dynamic processes has been mostly obtained via fluorescence microscopy such as total internal reflection and super-...
RegDate
2020-06-26
Hit
4725
Interferometric Scattering Microscopy with Polarization-Selective Detection Scheme
2019 IBS AOI Conference Single-molecule biophysical techniques have revolutionized the way to view biological systems. In particular, single-particle tracking methods can track individual biomolecules and reveal microscopic details of biological interactions that are often obscured by ensemble averaging. Tracking based on fluorescence detection is t...
RegDate
2020-06-24
Hit
2225
Quantitative detection of DNA methylation via the B-Z transition
2019 IBS AOI Conference DNA methylation is one of the most frequent and critical epigenetic modifications. Abnormal DNA methylation has been implicated in various health issues such as metabolic syndromes and a variety of cancers. It is therefore not only of fundamental interest but also of practical significance to probe the degree of DNA methylatio...
RegDate
2020-06-24
Hit
2091
Diffusion dynamics of fluorescent nano-diamonds in living cells detected by fluorescence-combined iSCAT system
2019 IBS AOI Conference Fluorescent nano-diamonds (fNDs) are promising photoluminescent probes due to embedded, perfectly photostable color centers. These carbon-based fluorescent nanoparticles became a suitable option for stable fluorescent probes in bioimaging applications because of their bright fluorescence, long fluorescence lifetime, no photoble...
RegDate
2020-06-15
Hit
3680
Time-gated iterative phase conjugation
2019 IBS AOI Conference In the field of optical imaging and therapy, where light is used to diagnose or treat disease, it is important to deliver sufficient light energy to a target within the biological tissues. However, the tissues give rise to multiple light scattering and spatial spread of the propagating waves as they are complex media composed...
RegDate
2020-06-15
Hit
2025
Dynamics of the time-gated reflection eigenchannel in homogeneous scattering medium
2019 IBS AOI Conference We investigate the dynamics of the time-gated reflection eigenchannels within homogeneous scattering medium. From the internal field distribution obtained from numerical simulations, we discovered the transition of the reflection enhancing mechanism as the target flight time of the time-gated reflection eigenchannel increases. Eige...
RegDate
2020-06-12
Hit
1775
Real-time microscopic recording of non-repetitive dynamic scenes at 5 THz frame
2019 IBS AOI Conference Femtoseconds-scale ultrafast imaging has served as an essential tool to directly visualize fast dynamics in a wide variety of applications in molecular biology, physical chemistry, atomic physics, and fluid dynamics. The conventional techniques providing a frame rate up to trillion frames per seconds include a streak camera, pu...
RegDate
2020-06-12
Hit
2258
Experimental mapping of near-field eigenmodes in sub-wavelength nanostructures
2019 IBS AOI Conference Near-field scanning optical microscopy (NSOM) is a useful tool for studying sub-diffraction nanostructures. As the size of nanostructures becomes smaller, the ability to observe and manipulate the near-field is getting more crucial. In ordinary NSOM imaging, the illumination of light source has not been a major concern since t...
RegDate
2020-06-11
Hit
3127
Aberration-free and flexible bundle-fiber endomicroscopy
2019 IBS AOI Conference The bundled fiber is commonly used as a probe of endoscopes due to its small dimension, flexibility and relatively large numerical aperture (NA). However, image pixelation and sample-induced aberration degrade spatial resolving power. T-matrix measurement has been proposed to resolve such problems, but it is vulnerable for th...
RegDate
2020-06-11
Hit
2467
Model eye imaging by closed-loop accumulation of single scattering (CLASS) microscopy
2019 Advances in Optics for Biotechnology, Medicine and Surgery XVII(An ECI Conference Series) ‘Closed-loop accumulation of single scattering (CLASS)’ microscopy provides novel solutions to the problems of light scattering and aberration in optical imaging, providing increased imaging depth while maintaining diffraction limited resolution. This me...
RegDate
2020-04-27
Hit
1721
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