- Marianne Chang
- Published 2022.08.30 12:18
A joint research team, including the Catholic University of Korea, Catholic Medical Center (CMC), has developed a holographic microscope to observe a living mouse’s brain neural networks at 3D high resolution without removing its skull.
Professor Kim Moon-seok of the Catholic University of Korea, Catholic Medical Center led a study that developed a 3D time-resolved holographic microscope to observe deep biological tissues in the mouse’s brain without removing its skull.
Light visualizing deep body tissues requires accurate measurement of the reflected signal by transmitting sufficient light energy. However, observing biological tissues with light is difficult due to multiple scattering phenomena from hitting various cells and optical aberrations.
It is possible to select the reflected light (single scattering wave) by hitting the target object and correcting the wavefront distortion caused by aberration. However, multiple scattering waves cause interference but removing this increases the ratio of single scattering waves enabling deeper biological tissue observation.
The research team, led by Professor Kim Moon-seok of CMC, developed the first pyrolysis hologram microscope that can remove multiple scattering and simultaneously measure the intensity and phase of light. As a result, it succeeded in observing the neural network of living fish without incision surgery. Still, it failed to do so in mice with thicker skulls due to severe light distortion and multiple scattering.
Professor Choi Won-shik of Korea University, deputy head of the molecular spectroscopy and dynamics research group at the Institute for Basic Science, and Professor Choi Myung-hwan of the Department of Life Science at Seoul National University also jointly conducted the research.
The joint researchers devised a method to select only a single scattered wave using the characteristics of this wave with similar reflective waveforms even when light is inserted at various angles. They analyzed the eigenmode of a wave-transmitting substance and found a resonance state that maximizes reinforcement interference between the light waves.
Consequently, they collected 80 times more light in the neural network than before and selectively removed unnecessary signals, significantly increasing the ratio of single scattering waves. They corrected the distortion at depths impossible with conventional techniques and obtained high-resolution brain neural network images under the skull without fluorescent labeling with lasers and without removing the mouse skull.
"From basic principles to observing neural networks in mice, we worked with physical, life, and neuroscience talents to open up new avenues for cranial neuroimaging fusion," Professors Kim and Choi said. "We hope it will have a ripple effect on industries that require a variety of biomedical fusion studies and precision measurements, including brain neuroscience."
The study, “Through-skull brain imaging in vivo at visible wavelengths via dimensionality reduction adaptive-optimal microscopy,” was published online in the Science Advances journal.
출처 : KBR(http://www.koreabiomed.com)
Professor Kim Moon-seok of the Catholic University of Korea, Catholic Medical Center led a study that developed a 3D time-resolved holographic microscope to observe deep biological tissues in the mouse’s brain without removing its skull.
Light visualizing deep body tissues requires accurate measurement of the reflected signal by transmitting sufficient light energy. However, observing biological tissues with light is difficult due to multiple scattering phenomena from hitting various cells and optical aberrations.
It is possible to select the reflected light (single scattering wave) by hitting the target object and correcting the wavefront distortion caused by aberration. However, multiple scattering waves cause interference but removing this increases the ratio of single scattering waves enabling deeper biological tissue observation.
The research team, led by Professor Kim Moon-seok of CMC, developed the first pyrolysis hologram microscope that can remove multiple scattering and simultaneously measure the intensity and phase of light. As a result, it succeeded in observing the neural network of living fish without incision surgery. Still, it failed to do so in mice with thicker skulls due to severe light distortion and multiple scattering.
Professor Choi Won-shik of Korea University, deputy head of the molecular spectroscopy and dynamics research group at the Institute for Basic Science, and Professor Choi Myung-hwan of the Department of Life Science at Seoul National University also jointly conducted the research.
The joint researchers devised a method to select only a single scattered wave using the characteristics of this wave with similar reflective waveforms even when light is inserted at various angles. They analyzed the eigenmode of a wave-transmitting substance and found a resonance state that maximizes reinforcement interference between the light waves.
Consequently, they collected 80 times more light in the neural network than before and selectively removed unnecessary signals, significantly increasing the ratio of single scattering waves. They corrected the distortion at depths impossible with conventional techniques and obtained high-resolution brain neural network images under the skull without fluorescent labeling with lasers and without removing the mouse skull.
"From basic principles to observing neural networks in mice, we worked with physical, life, and neuroscience talents to open up new avenues for cranial neuroimaging fusion," Professors Kim and Choi said. "We hope it will have a ripple effect on industries that require a variety of biomedical fusion studies and precision measurements, including brain neuroscience."
The study, “Through-skull brain imaging in vivo at visible wavelengths via dimensionality reduction adaptive-optimal microscopy,” was published online in the Science Advances journal.
출처 : KBR(http://www.koreabiomed.com)
Marianne Chang mchang@docdocdoc.co.kr
http://www.koreabiomed.com/news/articleView.html?idxno=14493
