Center for Molecular Spectroscopy and Dynamics

ABC 2018

Coherent optical imaging has been a useful tool for imaging of living biological subject, but its applicability has been limited to the superficial layers or early developmental stages due to the specimen-induced aberration and multiple light scattering. We propose label-free adaptive optical coherence microscopy free from hardware feedback. Experimental setup is based on wide-field and time-gated interferometric microscopy. Wide-field configuration enables us to collect whole optical modes, thereby offering optimal correction of high-order aberration. Data acquisition is simplified by arithmetic adaptive optical correction by post processing. Owing to improved data acquisition speed, the proposed method is readily applicable for biomedical applications. As a prospective application, we performed label-free neuroimaging of a living zebrafish and visualized tomographic details of neural network.