Center for Relativistic Laser Science

7th Jilin-Korea-Waseda Alliance Annual Symposium

Super-resolution fluorescence microscopy overcomes the resolution limit by means of optics and spectroscopy. On the other hand, expansion microscopy uses physically expanded sample linked to swellable polymer matrix. The enlarged sample allows for overcoming the resolution limit under conventional fluorescence microscopy. Combining the two methods (i.e. super-resolution imaging of expanded samples) may result in sub-10-nm resolution. However, the swollen volume and resultant sparse labeling density makes it difficult to apply super-resolution microscopy to expanded samples. Herein, we aim to boost fluorescence intensity in expanded sample by using a signal amplification method based on the biotin-avidin interaction and then to perform imaging of subcellular structures with DNA points accumulation for imaging in nanoscale topography (DNA-PAINT). Signal amplification is for boosting the density of labeled fluorophores and DNA-PAINT is a modality of localized-based super-resolution microscopy that is relatively insensitive to the chemical environment of the sample. In this study, we demonstrate the efficiency of amplified signals quantitatively and enhanced spatial resolution from the combination of super-resolution microscopy and expansion microscopy.

KEYWORDS: Expansion Microscopy, Signal Amplification, DNA points accumulation for imaging in nanoscale topography (DNA-PAINT)

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