Single-particle Tracking with Interferometric Scattering Microscopy (iSCAT)
2017 119th KCS Meeting
We can observe microscopic world with optical microscope. As techniques for imaging are developed, we can get images of nano-sized particles and molecules with high spatial resolution. Unfortunately, super-resolution imaging techniques using fluorescent molecules suffer photobleaching or photoblinking because lifetime of fluorophore is limited. However, detecting nanoparticles using interference of scattered light by themselves, we can directly observe dynamics of nano-materials with high time resolution as well as overcome time limit of fluorescence imaging. In this work, we constructed optical system of interferometric scattering microscopy (iSCAT). Using iSCAT, we got images of gold nanoparticles with 40 nm diameter and analyzed time trace of their movement.