Center for Molecular Spectroscopy and Dynamics

    IBS Center for Molecular Spectroscopy and Dynamics

■        SPEAKER
Prof. Ryota Iino (Institute for Molecular Science, National Institutes of Natural Sciences, Japan)

■        TITLE
High-speed single-molecule imaging analysis of protein molecular motors with plasmonic nanoprobes

■        ABSTRACT
We have been developing high-speed single-molecule imaging methods monitoring fast motions of protein molecular motors with nanometer localization precision and microsecond temporal resolution [1]. Our methods are based on dark-field scattering imaging of plasmonic nanoprobes such as gold nanoparticles [2] and nanorods [3]. As probes of single-molecule imaging, the plasmonic nanoprobes have advantages over the commonly used ones such as fluorescent dyes and quantum dots, because much stronger signals can be obtained without suffering from photobleaching and blinking. In this seminar, I will discuss the mechanisms of chemo-mechanical coupling, unidirectionality, and coordination of linear molecular motors kinesin-1 [4] and chitinase, and rotary molecular motors F1-ATPase and V1-ATPase [5-7], revealed by our high-speed single-molecule imaging analysis. I will also introduce our recent approaches to achieve angstrom-level localization precision and multi-color imaging with plasmonic nanoprobes.

■        DATE AND VENUE
  April 24, 2018 (Tuesday, 4:00 - 5:00 pm)  

Seminar Room 116, KU R&D Center

■        LANGUAGE
English

■        INVITED BY
Prof. Seok-Cheol Hong

      *If you want to have dinner with Prof. Ryota Iino or discuss with him, please contact Prof.              Seok-Cheol Hong (hongsc@korea.ac.kr).