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Cisplatin fastens nucleosomal DNA under physiological salt conditions
2019.07 Single-molecule biophysics conference Cisplatin is one of the most potent anti-cancer drugs, displaying clinical activity against various tumors. It is known that cisplatin binds to DNA and induces local kinks[1], which can lead to cell death[2]. The working mechanism of cisplatin has, however, not been fully understood yet. It is accepted that...
RegDate
2021-04-22
Hit
1630
Nuclease-free detection of DNA methylation via the B-Z transition
2019.07 Single-molecule biophysics conference DNA methylation is one of the most frequent and critical epigenetic modifications. Abnormal DNA methylation has been implicated in various health issues such as metabolic syndromes and a variety of cancers(1). It is therefore not only of fundamental interest but also of practical significance to probe the d...
RegDate
2021-04-22
Hit
3272
Z sectioning of intracellular organelles with remote-focusing-enabled iSCAT microscopy
2019.07 Single-molecule biophysics conference Interferometric scattering (iSCAT) microscopy can localize nanoscopic objects such as nanoparticles1,4, single protein molecules,3 and biomolecular components in the cells5 with high spatial precision. It also enables us to observe the spatial organization of subcellular organelles, dynamical behaviors an...
RegDate
2021-04-22
Hit
1673
Label-free and aberration-corrected endomicroscopic imaging using GRIN lens
Optics and Photonics Congress 2019 Abstract: The intrinsic aberration of the GRIN lenses makes it hard to obtain high resolution image. Recently, adaptive optics is widely used to correct the aberration, but it requires additional wave-front shaping devices and fluorescent label. Here, we present a label-free and aberration-corrected GRIN lens endosmi...
RegDate
2021-02-25
Hit
1813
Time-gated iterative phase conjugation of reflected wave
Optics and Photonics Congress 2019 Abstract: Light energy needs to be delivered efficiently to a target object for optical bioimaging and photo-therapy. However, multiple light scattering in biological tissues reduce light energy delivered to a target object by means of wave diffusion. Here, we propose an iterative phase conjugation of the time-gated...
RegDate
2021-02-25
Hit
1859
Label-free myelin imaging of a living zebrafish by simultaneous angular scanning microscopy
Optics and Photonics Congress 2019 Abstract: We presented collective accumulation of single scattering (CASS)1 microscopy and closed-loop accumulation of single scattering (CLASS)2 microscopy. However, its application to in vivo imaging has remained unrealistic so far to the long acquisition time of the time-gated reflection matrix. To speed up the ...
RegDate
2021-01-12
Hit
1673
Two-color resonant CARS microscopy for an interfacial dip-free detection
2019 RamanFest Abstract:In CARS imaging, with a non-resonant background, the dark dip exhibits at the interface between the objects and the medium. Although a variety of CARS imaging techniques have been already developed to eliminate a non-resonant contribution to the CARS signal, it still remains a promising task to remove such interfacial dip at ...
RegDate
2020-11-06
Hit
1614
Dual-Frequency Comb Nonlinear Spectroscopy: Direct Measurement of Correlation between Molecular Structure and Reaction Kinetics
2019 ICUSD Time-resolved spectroscopy has been revolutionarily developed in last four decades to unveil the secrets of complex photochemical systems in nature. In particular, femtosecond light source provides not only the femtosecond-time resolution but also the molecular structure of photochemical species. In these days, to obtain those information, ...
RegDate
2020-11-05
Hit
1769
Complementarily balanced real-time two-color SRS
2019 IBS AOI Conference The stimulated Raman scattering (SRS) utilizing inherent chemical contrast of sample is applicable to the label-free imaging with enhanced Raman signal intensity and chemical selectivity. It has been widely employed to investigate biomolecules and its dynamics in living organisms without perturbation of exogenous labels1. H...
RegDate
2020-10-27
Hit
1724
Single-molecule Localization Expansion Microscopy
2019 IBS AOI Conference Expansion Microscopy (ExM) is a method to improve the spatial resolution by physically expanding a sample linked to swellable gel. An advantage of the expansion microscopy is that it allows observers to overcome the diffraction limit using the conventional fluorescence microscopy. On the other hand, single-molecule localizat...
RegDate
2020-10-27
Hit
1695
Bright Ligand-activatable Fluorescent Protein for High-quality Multicolor Live-cell Super-resolution Microscopy
2019 IBS AOI Conference Multicolor single-molecule localization microscopy with fluorescent proteins is challenging due to the lack of bright photoswitchable fluorescent proteins in different spectral regions other than the orange spectrum. Here, we introduce UnaG as a green-to-dark photoswitching fluorescent protein capable of high-quality super-res...
RegDate
2020-10-27
Hit
1657
Deep tissue space-gated microscopy via acousto-optic interaction
2019 IBS AOI ConferenceConventional imaging scheme, relying on a ballistic wave, is severely compromised when applied to a multiply scattering medium. To mitigate the effect, a typical approach has been to distinguish and reject a multiply scattered wave in a time domain using a time-gating and/or coherence-gating method. Here, we present a new gating...
RegDate
2020-07-23
Hit
1621
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