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IBS Institute for Basic Science

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Reversible Fluorescence Switching of Bilirubin-bound UnaG Protein for Super-resolution Microscopy 2018 BPS18 UnaG is a fluorogen-binding protein which only fluoresces upon binding of a small metabolite molecule, bilirubin (BR). Here we introduce a photo-switchable nature of UnaG originated from repetitive binding of BR under continuous light illumination. We found that the on-/off-switching rates are mainly controlled by the concentration of BR and... RegDate 2019-11-13 Hit 1731
Interferometric scattering microscopy with polarization selective dual detection scheme: capturing the orienational information of anisotropic nanometr... 2018 BPS18 Single-particle tracking is a powerful technique to reveal an underlying principle of microscopic phenomena. Here we report an optical microscopy technique, polarization-selective interferometric scattering microscopy that enables us to capture rotational as well as positional information of nano-scale objects. This technique grants all the meri... RegDate 2019-11-13 Hit 1799
Maximal energy coupling to complex plasmonic devices by injecting light into eigenchannels 2018 SPIE Abstract― We present a systematic method to maximize the coupling of far-field waves to SPPs for arbitrary complex metallic nanostructures that do not have any band-gap structures. In the proposed method, we measure the transfer matrix of a given complex plasmonic device that connects far-field input to the SPP output. By performing the sin... RegDate 2019-11-13 Hit 1731
Fluorescence imaging of formation of triple helical DNA ICAMD 2017 Triple helical DNA (DNA triplex) is an exotic conformation of DNA, which is known to exist for special sequences under specific pH and salt conditions. In vivo formation of DNA triplex is thought to be responsible for a certain genetic disease such as Friedreich Ataxia. This conformation has also drawn much attention over decades because of ... RegDate 2019-11-05 Hit 2046
Label-free observation of cytoplasmic organelle structures using interferometric scattering microscopy ICAMD 2017 Interferometric scattering (iSCAT) microscopy is a new optical imaging technique, recently developed to identify the nano-sized particles without a fluorescent label. While the previous iSCAT studies have been mostly focused on the fast tracking of these particles moving in artificial bio-mimicking systems, we here report on label-free iSCAT... RegDate 2019-11-05 Hit 1986
Polarization-selective interferometric detection of nano objects ICAMD 2017 Single particle tracking is a powerful technique to reveal an underlying principle of microscopic phenomena. Here we report an optical microscopy technique, POlarization-SElective Interferometric Detection for Optical scattering Nanoscopy (POSEIDON), that enables us to capture rotational as well as positional information of nano-size object... RegDate 2019-11-05 Hit 1723
Super-resolved Expansion Microscopy 2017 KPS Fall Meeting Super-resolution fluorescence microscopy is a powerful imaging technique that overcome the diffraction limit by means of optics and spectroscopy. Expansion microscopy has also recently emerged as a novel technique improving resolution, but dependent on physically expanded sample linked to swellable polymer matrix. The enlarged s... RegDate 2019-11-05 Hit 2142
A New Photoswitchable Fluorescence Protein for Long-term Live-cell Super-resolution Imaging of Various Subcellular Structures 2017 KPS Fall Meeting Here we report a new way to image subcellular structures with sub-diffraction limit resolution by introducing a fluorogen inducible fluorescent protein, UnaG, whose fluorescence arise only when the protein binds to bilirubin (BR), a nonfluorescent metabolite. UnaG showed an efficient photoswitching property due to the no fluoresce... RegDate 2019-11-05 Hit 2340
The Optical Properties for Bilirubin-Inducible Fluorescent Protein (holoUnaG) 2017 120th KCS Meeting Bilirubin-inducible fluorescence protein (holoUnaG) was recently discovered and identified from eel muscle by the Miyawaki group. Fluorescence proteins like GFP have been used in a variety of biomedical researches. HoloUnaG of which size is smaller GFP is likely to be of use in studying biomedical and super-resolution researches.... RegDate 2019-10-31 Hit 2195
Vibrational Solvatochromism of Alkyne-Derivatized Infrared Probe 2017 120th KCS Meeting It is important to understand the solvatochromism of Raman imaging, because it can be used to observe small molecules by detecting molecular vibrations and has better spatial resolution than IR imaging. To observe the small molecules, recent Raman imaging experiments attach labels to target molecules, which is called Raman tag i... RegDate 2019-10-31 Hit 2216
Ultrafast exchange dynamics of electrolyte in Lithium ion battery 2017 120th KCS Meeting Electrolyte in lithium ion battery is mixture of lithium salt and carbonate solvents. Lithium ion is the charge carrier, carbonates form stable solvation sheath of Li+. Here two-dimensional infrared spectroscopy is applied to study dynamics of electrolyte in lithium ion battery. In LiPF6 diethylcarbonate(DEC) solution, 2DIR spec... RegDate 2019-10-31 Hit 2840
The relation between the Hofmeister anions and water structure at protein surfaces 2017 120th KCS Meeting Water has unique properties based on hydrogen bonding that is come from the specific atomic composition and molecular structure of water. These properties are crucial for the survival of life on the Earth. Thus, researched for water is highly important. Specially, we focused on these properties of water with protein and ions. To... RegDate 2019-10-31 Hit 2190

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